To cash in on all of the "real world" hype of the events in the first film, a man from Burkitsville, Maryland opens a "Blair Witch Hunt" tour, which shows patrons various locations from the original film. A bunch of college students decide to take the tour, and wind up in Rustin Parr's house. There, they decide to camp for the evening, but in the morning, they realize they didn't sleep and they don't remember anything that happened the previous night. From there, they go back to town, and discover that something...or someone has come with them.

Six months after the suicide of her twin sister Sofie, Megan still grieves her death and misses her beloved sister. In the Saint John's Eve, Megan travels with her friends Christian, Zoe, Mark and Alicia to her family's house in Nowell Lake, Louisiana, to celebrate the summer solstice. While shopping supplies in a local store, Megan befriends the seller Nick and buys a magazine with an article about communication with the dead in the summer solstice, the time of year when there is the greatest length of daylight. While in her house, Megan is haunted by a spirit that she believes is Sofie trying to communicate with her. In her investigation, she suspects of the weird hick Leonard and while snooping in his house, she finds the picture of the missing girl Malin and unravels a dark secret about the suicide of her sister.